Table 2.

In vitro cell viability studies

Cell lineBCNU aloneBCNU + low hCRF (0.01 nmol/L)BCNU + high hCRF (1.0 nmol/L)BCNU + low DEX (0.01 mmol/L)BCNU + high DEX (1.0 mmol/L)
A. hCRF and DEX effects on BCNU EC50 (μmol/L)
 U87226 ± 56 (9)264 ± 91 (9)193 ± 54 (9)311 ± 65 (9)a 260 ± 59 (9)
 C6414 ± 43 (8)419 ± 33 (8)388 ± 59 (8)346 ± 94 (8)304 ± 102 (9)a
 Ln229234 ± 43 (7)261 ± 58 (7)219 ± 36 (7)239 ± 47 (7)201 ± 91 (7)
 RG2140 ± 22 (6)259 ± 96 (6)173 ± 60 (6)329 ± 165 (6)a 147 ± 72 (6)
Cell lineTMZ aloneTMZ + low hCRF (0.01 nmol/L)TMZ + high hCRF (1.0 nmol/L)TMZ + low DEX (0.01 mmol/L)TMZ + high DEX (1.0 mmol/L)
B. hCRF and DEX effects on TMZ EC50 (mmol/L)
 U874.8 ± 3.5 (5)3.1 ± 1.8 (5)3.8 ± 2.8 (5)4.5 ± 3.3 (5)3.9 ± 3.2 (5)
 C62.9 ± 1.1 (5)3.6 ± 1.7 (5)2.9 ± 1.0 (5)4.5 ± 3.0 (5)3.8 ± 3.5 (5)
 Ln2293.1 ± 0.6 (6)3.2 ± 0.5 (6)3.3 ± 0.6 (6)3.4 ± 1.0 (6)3.5 ± 1.0 (6)
 RG29.8 ± 2.8 (5)6.9 ± 1.4 (5)*6.2 ± 0.3 (5)a 7.8 ± 2.1 (5)7.9 ± 1.6 (5)

Abbreviations: DEX, dexamethasone; TMZ, temozolomide.

  • aAssays were conducted after 24-hour exposure to the drugs. Significant difference compared with BCNU or temozolomide alone; P < 0.05 after adjustment for multiple comparisons using Dunnett's method.